Part:BBa_K3773522:Experience

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iGEM21_William_and_Mary

Our initial confirmation of this part's function was as follows. Once we confirmed the transformation of the circuit, we tested its functionality by measuring its fluorescence compared to untransformed cells and successfully fluorescing cells. When these tests showed that the circuit was not fluorescing above the levels of the untransformed cells, we set about inducing the SOS response in the cells to derepress LexA from the circuit’s ftsLp2 promoter. The transformed cells were exposed to UV light and subinhibitory levels of Ciprofloxacin (Sassanfar et al, 1990, Baharoglu et al, 2011), but all attempts were unsuccessful at making induced, transformed cells consistently and significantly more fluorescent than untransformed cells.

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